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  5.     <title>UTas ePrints - Isolation, amplification, and identification of ancient copepod DNA from lake sediments</title>
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  32. <meta content="Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
  33. features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a
  34. method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed
  35. in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
  36. sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
  37. Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
  38. Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
  39. in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments
  40. from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples
  41. stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol." name="eprints.abstract" />
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  51. <meta content="Altschul, S. F., T. L. Madden, A. A. Schaffer, J. H. Zhang,
  52. Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST
  53. and PSI-BLAST: a new generation of protein database search
  54. programs. Nucleic Acids Res. 25:3389-3402.
  55. Armstrong, T. K. C., L. G. C. Girouard, and T. J. Anchordoquy.
  56. 2002. Effects of PEGylation on the preservation of cationic
  57. lipid/DNA complexes during freeze-thawing and
  58. lyophilization. J. Pharm. Sci. 91:2549-2558.
  59. Bissett et al. Ancient copepod DNA
  60. 540
  61. Bayly, I. A. E., J. A. E. Gibson, B. Wagner, and K. M. Swadling.
  62. 2003. Taxonomy, ecology and zoogeography of two Antarctic
  63. freshwater calanoid copepod species: Boeckella poppei
  64. and Gladioferens antarcticus. Antarct. Sci. 15:439-448.
  65. Cohen, A. S. 2003. Paleolimnology: the history and evolution
  66. of lake systems. Oxford University Press.
  67. Cole, J. R., and others. 2005. The Ribosomal Database Project
  68. (RDP-II): sequences and tools for high-throughput rRNA
  69. analysis. Nucleic Acids Res. 33 (Database Issue):D294-D296.
  70. Coolen, M. J. L., and J. Overmann. 1998. Analysis of subfossil
  71. molecular remains of purple sulfur bacteria in a lake sediment.
  72. Appl. Environ. Microbiol. 64:4513-4521.
  73. ———, E. C. Hopmans, W. I. C. Rijpstra, G. Muyzer,
  74. S. Schouten, J. K. Volkman, and J. S. Sinninghe Damsté.
  75. 2004a. Evolution of the methane cycle in Ace Lake (Antarctica)
  76. during the Holocene: response of methanogens and
  77. methanotrophs to environmental changes. Org. Geochem.
  78. 35:1151-1167.
  79. ———, G. Muyzer, W. I. C. Rijpstra, S. Schouten, J. K. Volkman,
  80. and J. S. Sinninghe Damsté. 2004b. Combined DNA and
  81. lipid analyses of sediments reveal changes in Holocene
  82. haptophyte and diatom populations in an Antarctic lake.
  83. Earth Planet. Sci. Lett. 223:225-239.
  84. Cooper, A., and H. N. Poinar 2000. Ancient DNA: do it right or
  85. not at all. Science 289:1139.
  86. Cousyn, C., L. De Meester, J. K. Colbourne, J. K. Brendonck, D.
  87. Verschuren, and F. Volckaert. 2001. Rapid, local adaptation
  88. of zooplankton behavior to changes in predation pressure
  89. in the absence of neutral genetic changes. Proc. Natl. Acad.
  90. Sci. U S A 98:6256-6260.
  91. Cromer, L., J. A. E. Gibson, K. M. Swadling, and D. A. Hodgson.
  92. 2005a. Evidence for a faunal refuge in the Larsemann Hills,
  93. East Antarctica, during the Last Glacial Maximum. Quaternary
  94. Australasia 22:12-13.
  95. ———, J. A. E. Gibson, K. M. Swadling, and D. A. Ritz. 2005b.
  96. Faunal microfossils: indicators for ecological change in an
  97. Antarctic saline lake. Palaeogeogr. Palaeoclimatol. Palaeoecol.
  98. 221:83-97.
  99. Dartnall, H. J. G. 1995. Rotifers, and other aquatic invertebrates,
  100. from the Larsemann Hills, Antarctica. Pap. Proc. Roy.
  101. Soc. Tasmania 129:17-23.
  102. Duffy, M. A., L. J. Perry, C. M. Kearns, L. J. Weider, and N. G. Hairston.
  103. 2000. Paleogenetic evidence for a past invasion of
  104. Onondaga Lake, NY, by exotic Daphnia curvirostris using
  105. mtDNA from dormant eggs. Limnol. Oceanogr. 45:1409-1414.
  106. Frey, D.G. 1964. Remains of animals in Quaternary lake and
  107. bog sediments and their interpretation. Arch. Hydrobiol.
  108. Beih. Ergebn. Limnol. 2:1-114.
  109. Hairston, N. G., Jr. 1996. Zooplankton egg banks as biotic
  110. reservoirs in changing environments. Limnol. Oceanogr.
  111. 41:1087-1092.
  112. ——— and A. J. Bohonak. 1998. Copepod reproductive strategies:
  113. life-history theory, phylogenetic pattern and invasion
  114. of inland waters. J. Mar. Systems 15:23-34.
  115. ——— and C. E. Cáceres. 1996. Distribution of crustacean diapause:
  116. micro- and macroevolutionary pattern and process.
  117. Hydrobiologia 320:27-44.
  118. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence
  119. alignment editor and analysis program for Windows
  120. 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
  121. Hebert, P. D. N., A. Cywinska, S. L. Ball, and J. R. deWaard.
  122. 2003. Biological identifications through DNA barcodes.
  123. Proc. Roy. Soc. Lond. B 270:313-322.
  124. Hodgson, D. A., P. E. Noon, and others. 2001. Were the Larsemann
  125. Hills ice-free through the Last Glacial Maximum?
  126. Antarct. Sci. 13:440-454.
  127. ———, E. Verleyen, and others. 2005. Late Quaternary
  128. climate-driven environmental change in the Larsemann
  129. Hills, East Antarctica: multi-proxy evidence from a lake
  130. sediment core. Quaternary Res. 64:83-99.
  131. Huys, R., and G. A. Boxshall. 1991. Copepod evolution. The
  132. Ray Society, London.
  133. Jarman, S. N. 2004. Amplicon: software for designing PCR
  134. primers on aligned DNA sequences. Bioinform. 20:
  135. 1644-1645.
  136. ———, B. E. Deagle, and N. J. Gales. 2004. Group-specific
  137. polymerase chain reaction for DNA-based analysis of
  138. species identity and diversity in dietary samples. Mol. Ecol.
  139. 13:1313-1322.
  140. Khanna, M., and G. Stotzky. 1992. Transformation of Bacillus
  141. subtilis by DNA bound on montmorillonite and effect of
  142. DNase on the transforming ability of bound DNA. Appl.
  143. Environ. Microbiol. 58:1930-1939.
  144. Leavitt, P. R., and D. A. Hodgson. 2001. Sedimentary pigments.
  145. In J. P. Smol, H. J. B. Birks and W. M. Last [eds.],
  146. Tracking environmental change using lake sediments. Volume
  147. 3: Terrestrial, algal and siliceous indicators. Kluwer.
  148. Limburg, P. A., and L. J. Weider. 2002. ‘Ancient’ DNA in the
  149. resting egg bank of a microcrustacean can serve as a palaeolimnological
  150. database. Proc. Roy. Soc. London B 269:
  151. 281-287.
  152. Lorenz, M. G., and W. Wackernagel. 1987. Adsorption of DNA
  153. to sand and variable degradation rates of adsorbed DNA.
  154. Appl. Environ. Microbiol. 53:2948-2952.
  155. Meyers, P. A. 1997. Organic geochemical proxies for
  156. palaeooceanographic, paleolimnologic, and paleoclimatic
  157. processes. Org. Geochem. 27:213-250.
  158. ——— and J. L. Teranes. 2001. Sediment organic matter,
  159. p. 239-269. In W. M. Last and J. P. Smol [eds.], Tracking
  160. environmental change using lake sediments. Volume 2:
  161. Physical and geochemical methods. Kluwer.
  162. McCormac, F. G., A. G. Hogg, P. G. Blackwell, C. E. Buck,
  163. T. F. G. Higham, and P. J. Reimer. 2004. SHCal04 Southern
  164. Hemisphere Calibration 0-11000 cal BP. Radiocarbon 46:
  165. 1087-1092.
  166. Page, R. D. M. 1996. TREEVIEW: an application to display phylogenetic
  167. trees on personal computers. Comput. Appl. Biosci.
  168. 12:357-358.
  169. Bissett et al. Ancient copepod DNA
  170. 541
  171. Rautio, M., S. Sorvari, and A. Korhola. 2000. Diatom and crustacean
  172. zooplankton communities, their seasonal variability
  173. and their representation in the sediment of subarctic Lake
  174. Saanajärvi. J. Limnol. 59 (Suppl. 1):81-96.
  175. Reid, V. A., G. R. Carvalho, and D. G. George. 2000. Molecular
  176. genetic analysis of Daphnia in the English Lake District:
  177. species identity, hybridization and resting egg banks.
  178. Freshw. Biol. 44:247-253.
  179. ———, G. R. Carvalho, D. G. George, and H. I. Griffiths. 2002.
  180. A technique for the molecular genetic analysis of Daphnia
  181. resting eggs from sub-recent lake sediments. J. Paleolimnol.
  182. 27:481-486.
  183. Smol, J. P., H. J. B. Birks, and W. M. Last. 2001. Tracking environmental
  184. change using lake sediments. Volume 4: Zoological
  185. indicators. Kluwer.
  186. ———, A. P. Wolfe, and others. 2005. Climate-driven regime
  187. shifts in the biological communities of arctic lakes. Proc.
  188. Natl. Acad. Sci. U S A 102:4397-4402.
  189. Stuiver, M., and P. J. Reimer. 1993. Extended 14C database and
  190. revised CALIB radiocarbon calibration program. Radiocarbon
  191. 35:215-230.
  192. ———, P. J. Reimer, and R. Reimer. 2005. CALIB Radiocarbon Calibration.
  193. Version 5.0.2. http://radiocarbon.pa.qub.ac.uk/calib/.
  194. Swadling, K. M., A. D. McKinnon, G. De’ath, and J. A. E. Gibson.
  195. 2004. Growth, production and life cycle plasticity of the
  196. copepod Paralabidocera antarctica in east Antarctica. Limnol.
  197. Oceanogr. 49:644-655.
  198. Tautz, D., P. Arctander, A. Minelli, R. H. Thomas, and A. P.
  199. Vogler. 2003. A plea for DNA taxonomy. Trends Ecol. Evol.
  200. 18:70-74.
  201. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin,
  202. and D. G. Higgins. 1997. The ClustalX windows interface:
  203. flexible strategies for multiple sequence alignment
  204. aided by quality analysis tools. Nucleic Acids Res. 25:
  205. 4876-4882.
  206. Tippet, R. 1964. An investigation into the nature of the layering
  207. of deepwater sediments in two eastern Ontario lakes.
  208. Can. J. Bot. 4:1693-1708.
  209. Wagner, B., H. Cremer, N. Hultzsch, D. B. Gore, and M. Melles.
  210. 2004. Late Pleistocene and Holocene history of Lake Terrasovoje,
  211. Amery Oasis, East Antarctica, and its climatic and
  212. environmental implications. J. Paleolimnol. 32:321-339.
  213. Warner, B. G. 1989. Other fossils. Geosci. Can. 16:231-242.
  214. Wayne, R. K., J. A. Leonard, and A. Cooper. 1999. Full of sound
  215. and fury: the recent history of ancient DNA. Annu. Rev.
  216. Ecol. Syst. 30:457-477.
  217. Willerslev, E., A. J. Hansen, and H. N. Poinar. 2004. Isolation
  218. of nucleic acids and cultures from fossil ice and permafrost.
  219. Trends Ecol. Evol. 19:141-147.
  220. Wuyts, J., P. De Rijk, Y. Van de Peer, T. Winkelmans, and R. De
  221. Wachter. 2001. The European large subunit ribosomal RNA
  222. database. Nucleic Acids Res. 29:175-177.
  223. Zale, R. 1994. 14C age corrections in antarctic lake sediments
  224. inferred from geochemistry. Radiocarbon 36:173-185.
  225. ——— and W. Karlén. 1989. Lake sediment cores from the
  226. Antarctic Peninsula and surrounding islands. Geogr. Ann. A
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  238. <meta content="Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic
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  241. in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA
  242. sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.
  243. Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.
  244. Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified
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  361.     <h1 class="ep_tm_pagetitle">Isolation, amplification, and identification of ancient copepod DNA from lake sediments</h1>
  362.     <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Bissett, Andrew</span> and <span class="person_name">Gibson, John A.E.</span> and <span class="person_name">Jarman, Simon N.</span> and <span class="person_name">Swadling, Kerrie M.</span> and <span class="person_name">Cromer, Louise</span> (2005) <xhtml:em>Isolation, amplification, and identification of ancient copepod DNA from lake sediments.</xhtml:em> Limnology and Oceanography: Methods, 3 . pp. 533-542. ISSN 1541-5856</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/701/1/Bissett.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" class="ep_doc_icon" border="0" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/701/1/Bissett.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />126Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input accept-charset="utf-8" value="714" name="docid" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://aslo.org/lomethods/free/2005/index.html">http://aslo.org/lomethods/free/2005/index.html</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Species identification of copepods in lake sediments is often difficult because their remains lack diagnostic&#13;
  363. features. It is therefore not easy to track changes in copepod biodiversity in lakes through time. We report a&#13;
  364. method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed&#13;
  365. in the early Holocene to the present. The method, which involves amplification of a short (~300 base pair) DNA&#13;
  366. sequence that varies between copepod species, provides a new approach to the study of copepod paleobiodiversity.&#13;
  367. Successful amplification of copepod DNA was possible in samples as old as 9950 calibrated 14C y BP.&#13;
  368. Attempts failed to recover DNA from a sediment sample ca 65,000 years old. In most cases the species identified&#13;
  369. in the sediments matched those of extant lake populations, but analysis of early-mid Holocene sediments&#13;
  370. from one lake revealed a species that is not present today. We were able to recover copepod DNA from core samples&#13;
  371. stored at -20 degrees C, at 4 degrees C, and preserved with polyethyleneglycol.</p></div><table style="margin-bottom: 1em" cellpadding="3" class="not_ep_block" border="0"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">ancient DNA, copepod, Antarctica, lake, Boeckella poppei, Paralabidocera antarctica</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270299.html">270000 Biological Sciences &gt; 270200 Genetics &gt; 270299 Genetics not elsewhere classified</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">701</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Dr John A.E. Gibson</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">08 Feb 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">08 Feb 2008 15:26</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=701;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&amp;eprintid=701">item control page</a></p>
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